Localization of telomeres and telomere-associated proteins in telomerase-negative Saccharomyces cerevisiae.

ABSTRACT: Cells lacking telomerase cannot maintain their telomeres and undergo a telomere erosion phase leading to senescence and crisis in which most cells become nonviable. On rare occasions survivors emerge from these cultures that maintain their telomeres in alternative ways. The movement of five marked telomeres in Saccharomyces cerevisiae was followed in wild-type cells and through erosion, senescence/crisis and eventual survival in telomerase-negative (est2::HYG) yeast cells. It was found that during erosion, movements of telomeres in est2::HYG cells were indistinguishable from wild-type telomere movements. At senescence/crisis, however, most cells were in G(2) arrest and the nucleus and telomeres traversed back and forth across the bud neck, presumably until cell death. Type I survivors, usi

SUBMITTER: Straatman KR 

PROVIDER: S-EPMC2784495 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature