U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis.

ABSTRACT:

Background

U3 snoRNA is a box C/D small nucleolar RNA (snoRNA) involved in the processing events that liberate 18S rRNA from the ribosomal RNA precursor (pre-rRNA). Although U3 snoRNA is present in all eukaryotic organisms, most investigations of it have focused on fungi (particularly yeasts), animals and plants. Relatively little is known about U3 snoRNA and its gene(s) in the phylogenetically broad assemblage of protists (mostly unicellular eukaryotes). In the euglenozoon Euglena gracilis, a distant relative of the kinetoplastid protozoa, Southern analysis had previously revealed at least 13 bands hybridizing with U3 snoRNA, suggesting the existence of multiple copies of U3 snoRNA genes.

Results

Through screening of a lambda genomic library and PCR amplification, we recove

SUBMITTER: Charette JM 

PROVIDER: S-EPMC2784804 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature