Characterisation of microRNA expression in post-natal mouse mammary gland development.

ABSTRACT:

Background

The differential expression pattern of microRNAs (miRNAs) during mammary gland development might provide insights into their role in regulating the homeostasis of the mammary epithelium. Our aim was to analyse these regulatory functions by deriving a comprehensive tissue-specific combined miRNA and mRNA expression profile of post-natal mouse mammary gland development.We measured the expression of 318 individual murine miRNAs by bead-based flow-cytometric profiling of whole mouse mammary glands throughout a 16-point developmental time course, including juvenile, puberty, mature virgin, gestation, lactation, and involution stages. In parallel whole-genome mRNA expression data were obtained.

Results

One third (n = 102) of all murine miRNAs analysed were detected duri

SUBMITTER: Avril-Sassen S 

PROVIDER: S-EPMC2784809 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature