Biophysical and structural characterization of a robust octameric beta-peptide bundle.

ABSTRACT: Proteins composed of alpha-amino acids are essential components of the machinery required for life. Stanley Miller's renowned electric discharge experiment provided evidence that an environment of methane, ammonia, water, and hydrogen was sufficient to produce alpha-amino acids. This reaction also generated other potential protein building blocks such as the beta-amino acid beta-glycine (also known as beta-alanine); however, the potential of these species to form complex ordered structures that support functional roles has not been widely investigated. In this report we apply a variety of biophysical techniques, including circular dichroism, differential scanning calorimetry, analytical ultracentrifugation, NMR and X-ray crystallography, to characterize the oligomerization of two 12-mer be

SUBMITTER: Goodman JL 

PROVIDER: S-EPMC2784816 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature