Genetic mapping of targets mediating differential chemical phenotypes in Plasmodium falciparum.

ABSTRACT: Studies of gene function and molecular mechanisms in Plasmodium falciparum are hampered by difficulties in characterizing and measuring phenotypic differences between individual parasites. We screened seven parasite lines for differences in responses to 1,279 bioactive chemicals. Hundreds of compounds were active in inhibiting parasite growth; 607 differential chemical phenotypes, defined as pairwise IC(50) differences of fivefold or more between parasite lines, were cataloged. We mapped major determinants for three differential chemical phenotypes between the parents of a genetic cross, and we identified target genes by fine mapping and testing the responses of parasites in which candidate genes were genetically replaced with mutant alleles. Differential sensitivity to dihydroergotamine m

SUBMITTER: Yuan J 

PROVIDER: S-EPMC2784992 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature