FACS-assisted microarray profiling implicates novel genes and pathways in zebrafish gastrointestinal tract development.

ABSTRACT:

Background & aims

The zebrafish Danio rerio is an excellent model system for mammalian gastrointestinal development. To identify differentially regulated genes important in gastrointestinal organogenesis, we profiled the transcriptome of the zebrafish developing gastrointestinal tract.

Methods

Embryos from a transgenic zebrafish line expressing green fluorescent protein (GFP) in the developing intestine, liver, and pancreas were dissociated at 4 developmental time points, their cells sorted based on GFP expression with fluorescence-activated cell sorting (FACS), and analyzed with microarrays. To improve our analysis, we annotated the Affymetrix Zebrafish GeneChip with human orthologs.

Results

Transcriptional profiling showed significant differences between GFP(+) and

SUBMITTER: Stuckenholz C 

PROVIDER: S-EPMC2785077 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature