Genome-wide identification of Schistosoma japonicum microRNAs using a deep-sequencing approach.

ABSTRACT:

Background

Human schistosomiasis is one of the most prevalent and serious parasitic diseases worldwide. Schistosoma japonicum is one of important pathogens of this disease. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs in a given organism is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and posttranscriptional regulation.

Methodology/principal findings

We sequenced two small RNA libraries prepared from different stages of the life cycle of S. japonicum, immature schistosomula and mature pairing adults, through a deep DNA sequencing approach, which yielded approximately 12 million high

SUBMITTER: Huang J 

PROVIDER: S-EPMC2785426 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature