NET37, a nuclear envelope transmembrane protein with glycosidase homology, is involved in myoblast differentiation.

ABSTRACT: The nuclear lamina and its associated proteins are important for nuclear structure and chromatin organization and also have been implicated in the regulation of cell signaling and gene expression. In this study we demonstrate that the lamina-associated nuclear envelope transmembrane protein NET37 is required for myogenic differentiation of C2C12 cells. NET37, a member of glycosidase family 31, is highly expressed in mouse skeletal muscle and is strongly up-regulated during C2C12 differentiation. By protease mapping we show that its glycosidase homology domain is located in the lumen of the nuclear envelope/endoplasmic reticulum. When NET37 is depleted from proliferating myoblasts by RNAi, myogenic differentiation is significantly impaired, and there is a concomitant delay in up-regulation

SUBMITTER: Datta K 

PROVIDER: S-EPMC2785598 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature