Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi.

ABSTRACT: The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

SUBMITTER: Imwong M 

PROVIDER: S-EPMC2786678 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature