Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.

ABSTRACT: The interaction of proteins at cellular interfaces is critical for many biological processes, from intercellular signaling to cell adhesion. For example, the selectin family of adhesion receptors plays a critical role in trafficking during inflammation and immunosurveillance. Quantitative measurements of binding rates between surface-constrained proteins elicit insight into how molecular structural details and post-translational modifications contribute to function. However, nano-scale transport effects can obfuscate measurements in experimental assays. We constructed a biophysical simulation of the motion of a rigid microsphere coated with biomolecular adhesion receptors in shearing flow undergoing thermal motion. The simulation enabled in silico investigation of the effects of kinetic fo

SUBMITTER: Schmidt BJ 

PROVIDER: S-EPMC2787012 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature