Disparate oxidant gene expression of airway epithelium compared to alveolar macrophages in smokers.

ABSTRACT:

Background

The small airway epithelium and alveolar macrophages are exposed to oxidants in cigarette smoke leading to epithelial dysfunction and macrophage activation. In this context, we asked: what is the transcriptome of oxidant-related genes in small airway epithelium and alveolar macrophages, and does their response differ substantially to inhaled cigarette smoke?

Methods

Using microarray analysis, with TaqMan RT-PCR confirmation, we assessed oxidant-related gene expression in small airway epithelium and alveolar macrophages from the same healthy nonsmoker and smoker individuals.

Results

Of 155 genes surveyed, 87 (56%) were expressed in both cell populations in nonsmokers, with higher expression in alveolar macrophages (43%) compared to airway epithelium (24%). I

SUBMITTER: Carolan BJ 

PROVIDER: S-EPMC2787510 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature