Transcriptome and proteome exploration to model translation efficiency and protein stability in Lactococcus lactis.

ABSTRACT: This genome-scale study analysed the various parameters influencing protein levels in cells. To achieve this goal, the model bacterium Lactococcus lactis was grown at steady state in continuous cultures at different growth rates, and proteomic and transcriptomic data were thoroughly compared. Ratios of mRNA to protein were highly variable among proteins but also, for a given gene, between the different growth conditions. The modeling of cellular processes combined with a data fitting modeling approach allowed both translation efficiencies and degradation rates to be estimated for each protein in each growth condition. Estimated translational efficiencies and degradation rates strongly differed between proteins and were tested for their biological significance through statistical correlatio

SUBMITTER: Dressaire C 

PROVIDER: S-EPMC2787624 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature