Identification of molecular distinctions between normal breast-associated fibroblasts and breast cancer-associated fibroblasts.

ABSTRACT: Stromal fibroblasts influence the behavior of breast epithelial cells. Fibroblasts derived from normal breast (NAF) inhibit epithelial growth, whereas fibroblasts from breast carcinomas (CAF) have less growth inhibitory capacity and can promote epithelial growth. We sought to identify molecules that are differentially expressed in NAF versus CAF and potentially responsible for their different growth regulatory abilities. To determine the contribution of soluble molecules to fibroblast-epithelial interactions, NAF were grown in 3D, transwell or direct contact co-cultures with MCF10AT epithelial cells. NAF suppressed proliferation of MCF10AT in both direct contact and transwell co-cultures, but this suppression was significantly greater in direct co-cultures, indicating involvement of both s

SUBMITTER: Sadlonova A 

PROVIDER: S-EPMC2787925 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature