Replication fork collisions cause pathological chromosomal amplification in cells lacking RecG DNA translocase.

ABSTRACT: Duplication and transmission of chromosomes require precise control of chromosome replication and segregation. Here we present evidence that RecG is a major factor influencing these processes in bacteria. We show that the extensive DnaA-independent stable DNA replication observed without RecG can lead to replication of any area of the chromosome. This replication is further elevated following irradiation with UV light and appears to be perpetuated by secondary events that continue long after the elimination of UV lesions. The resulting pathological cascade is associated with an increased number of replication forks traversing the chromosome, sometimes with extensive regional amplification of the chromosome, and with the accumulation of highly branched DNA intermediates containing few Holli

SUBMITTER: Rudolph CJ 

PROVIDER: S-EPMC2788051 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature