Profiling of high-grade central osteosarcoma and its putative progenitor cells identifies tumourigenic pathways.

ABSTRACT:

Background

Osteosarcoma is the most prevalent primary malignant bone tumour in children and young adults, with poor survival in 40% of patients. To identify the signalling pathways involved in tumourigenesis, we compared gene expression in osteosarcoma with that in its presumed normal counterparts.

Methods

Genome-wide expression profiles were generated from 25 high-grade central osteosarcoma prechemotherapy biopsies, 5 osteoblastomas, 5 mesenchymal stem cell (MSC) populations and these same MSCs differentiated into osteoblasts. Genes that were differentially expressed were analysed in the context of the pathways in which they function using the GenMAPP programme.

Results

MSCs, osteoblasts, osteoblastomas and osteosarcomas clustered separately and thousands of differen

SUBMITTER: Cleton-Jansen AM 

PROVIDER: S-EPMC2788255 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature