BOAT: Basic Oligonucleotide Alignment Tool.

ABSTRACT:

Background

Next-generation DNA sequencing technologies generate tens of millions of sequencing reads in one run. These technologies are now widely used in biology research such as in genome-wide identification of polymorphisms, transcription factor binding sites, methylation states, and transcript expression profiles. Mapping the sequencing reads to reference genomes efficiently and effectively is one of the most critical analysis tasks. Although several tools have been developed, their performance suffers when both multiple substitutions and insertions/deletions (indels) occur together.

Results

We report a new algorithm, Basic Oligonucleotide Alignment Tool (BOAT) that can accurately and efficiently map sequencing reads back to the reference genome. BOAT can handle several

SUBMITTER: Zhao SQ 

PROVIDER: S-EPMC2788372 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature