Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS.

ABSTRACT:

Background

Dendritic cells (DCs) are the sentinels of the mammalian immune system, characterized by a complex maturation process driven by pathogen detection. Although multiple studies have described the analysis of activated DCs by transcriptional profiling, recent findings indicate that mRNAs are also regulated at the translational level. A systematic analysis of the mRNAs being translationally regulated at various stages of DC activation was performed using translational profiling, which combines sucrose gradient fractionation of polysomal-bound mRNAs with DNA microarray analysis.

Results

Total and polysomal-bound mRNA populations purified from immature, 4 h and 16 h LPS-stimulated human monocyte-derived DCs were analyzed on Affymetrix microarrays U133 2.0. A group of 375

SUBMITTER: Ceppi M 

PROVIDER: S-EPMC2788525 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature