Developmental expression of a functional TASK-1 2P domain K+ channel in embryonic chick heart.

ABSTRACT:

Background

Background K+ channels are the principal determinants of the resting membrane potential (RMP) in cardiac myocytes and thus, influence the magnitude and time course of the action potential (AP).

Methods

RT-PCR and in situ hybridization are used to study the distribution of TASK-1 and whole-cell patch clamp technique is employed to determine the functional expression of TASK-1 in embryonic chick heart.

Results

Chicken TASK-1 was expressed in the early tubular heart, then substantially decreased in the ventricles by embryonic day 5 (ED5), but remained relatively high in ED5 and ED11 atria. Unlike TASK-1, TASK-3 was uniformly expressed in heart at all developmental stages. In situ hybridization studies further revealed that TASK-1 was expressed throughout myoca

SUBMITTER: Zhang H 

PROVIDER: S-EPMC2788539 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature