Human cytomegalovirus UL76 induces chromosome aberrations.

ABSTRACT:

Background

Human cytomegalovirus (HCMV) is known to induce chromosome aberrations in infected cells, which can lead to congenital abnormalities in infected fetuses. HCMV UL76 belongs to a conserved protein family from herpesviruses. Some reported roles among UL76 family members include involvement in virulence determination, lytic replication, reactivation of latent virus, modulation of gene expression, induction of apoptosis, and perturbation of cell cycle progression, as well as potential nuclease activity. Previously, we have shown that stable expression of UL76 inhibits HCMV replication in glioblastoma cells.

Methods

To examine chromosomal integrity and the DNA damage signal gamma-H2AX in cells constitutively expressing UL76, immunofluorescent cell staining and Western b

SUBMITTER: Siew VK 

PROVIDER: S-EPMC2788540 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature