Lung mast cells are a source of secreted phospholipases A2.

ABSTRACT:

Background

Secreted phospholipases A(2) (sPLA(2)s) are released in plasma and other biologic fluids of patients with inflammatory, autoimmune, and allergic diseases.

Objective

We sought to evaluate sPLA(2) activity in the bronchoalveolar lavage fluid (BALF) of asthmatic patients and to examine the expression and release of sPLA(2)s from primary human lung mast cells (HLMCs).

Methods

sPLA(2) activity was measured in BALF and supernatants of either unstimulated or anti-IgE-activated HLMCs as hydrolysis of oleic acid from radiolabeled Escherichia coli membranes. Expression of sPLA(2)s was examined by using RT-PCR. The release of cysteinyl leukotriene (LT) C(4) was measured by means of enzyme immunoassay.

Results

Phospholipase A(2) (PLA(2)) activity was higher in t

SUBMITTER: Triggiani M 

PROVIDER: S-EPMC2788562 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature