Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells.

ABSTRACT: Interstrand cross-links (ICLs) are absolute blocks to transcription and replication and can provoke genomic instability and cell death. Studies in bacteria define a two-stage repair scheme, the first involving recognition and incision on either side of the cross-link on one strand (unhooking), followed by recombinational repair or lesion bypass synthesis. The resultant monoadduct is removed in a second stage by nucleotide excision repair. In mammalian cells, there are multiple, but poorly defined, pathways, with much current attention on repair in S phase. However, many questions remain, including the efficiency of repair in the absence of replication, the factors involved in cross-link recognition, and the timing and demarcation of the first and second repair cycles. We have followed the

SUBMITTER: Muniandy PA 

PROVIDER: S-EPMC2788842 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature