A comparative analysis of gene expression patterns and cell phenotypes between cervical and peripheral blood mononuclear cells.

ABSTRACT: Studies of the immunological environment in the female genital tract (FGT) are critical for the development of vaccines or microbicides to halt the spread of sexually transmitted infections. Challenges arise due to the difficulties of sampling from this site, and the majority of studies have been conducted utilising peripheral blood mononuclear cells. Identifying functional differences between immune cells of the FGT and peripheral blood would aid in our understanding of mucosal immunology. We compared the gene expression profile of mononuclear cells at these two sites. Messenger RNA expression analysis was performed using gene expression arrays on matched cervical mononuclear cells and peripheral blood mononuclear cells. Further cellular phenotyping was done by 10 colour flow cytometry. O

SUBMITTER: Horton RE 

PROVIDER: S-EPMC2790076 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature