Surface plasmon resonance for high-throughput ligand screening of membrane-bound proteins.

ABSTRACT: Technologies based on surface plasmon resonance (SPR) have allowed rapid, label-free characterization of protein-protein and protein-small molecule interactions. SPR has become the gold standard in industrial and academic settings, in which the interaction between a pair of soluble binding partners is characterized in detail or a library of molecules is screened for binding against a single soluble protein. In spite of these successes, SPR is only beginning to be adapted to the needs of membrane-bound proteins which are difficult to study in situ but represent promising targets for drug and biomarker development. Existing technologies, such as BIAcoreTM, have been adapted for membrane protein analysis by building supported lipid layers or capturing lipid vesicles on existing chips. Newer t

SUBMITTER: Maynard JA 

PROVIDER: S-EPMC2790208 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature