Single nucleotide polymorphism discovery in rainbow trout by deep sequencing of a reduced representation library.

ABSTRACT:

Background

To enhance capabilities for genomic analyses in rainbow trout, such as genomic selection, a large suite of polymorphic markers that are amenable to high-throughput genotyping protocols must be identified. Expressed Sequence Tags (ESTs) have been used for single nucleotide polymorphism (SNP) discovery in salmonids. In those strategies, the salmonid semi-tetraploid genomes often led to assemblies of paralogous sequences and therefore resulted in a high rate of false positive SNP identification. Sequencing genomic DNA using primers identified from ESTs proved to be an effective but time consuming methodology of SNP identification in rainbow trout, therefore not suitable for high throughput SNP discovery. In this study, we employed a high-throughput strategy that used pyrose

SUBMITTER: Sanchez CC 

PROVIDER: S-EPMC2790473 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature