Influence of sex on gene expression in human corneal epithelial cells.

ABSTRACT:

Purpose

Sex-associated differences have been identified in the anatomy, physiology and pathophysiology of the human cornea. We hypothesize that many of these differences are due to fundamental variations in gene expression. Our objective in this study was to determine whether such differences exist in human corneal epithelial cells both in vivo and in vitro.

Methods

Human corneal epithelial cells were isolated from the corneoscleral rims of male and female donors. Cells were processed either directly for RNA extraction, or first cultured in phenol red-free keratinocyte serum-free media. The RNA samples were examined for differentially expressed mRNAs by using of CodeLink Bioarrays and Affymetrix GeneChips. Data were analyzed with GeneSifter.Net software.

Results

Our r

SUBMITTER: Suzuki T 

PROVIDER: S-EPMC2790476 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature