Induction of muscle weakness by local inflammation: an experimental animal model.

ABSTRACT:

Objective and design

The objective of this study was to characterize the response of skeletal muscle to a localized inflammation induced by the inflammatory agent casein.

Methods

An inflammatory agent, casein, was injected into the right hindlimb and saline was injected into the left hindlimb of normal adult mice, once daily for six consecutive days. Inflammatory response was monitored by immunohistochemical labeling of leukocytes. Muscle protein levels were determined by electrophoresis and muscle function was determined by isometric force measurements.

Results

Local inflammation was induced by casein in association with the accumulation of extensive neutrophils and macrophages in the soleus muscle. This local inflammation resulted in a shift in myosin heavy chain (M

SUBMITTER: Bicer S 

PROVIDER: S-EPMC2790866 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature