Trichostatin A enhances proliferation and migration of vascular smooth muscle cells by downregulating thioredoxin 1.

ABSTRACT:

Aims

A reduction in the level of thioredoxin 1 (Trx1) has been proposed as a possible mechanism for the tumor-specific growth arrest caused by inhibition of histone deacetylases (HDACs). In this study, we investigated the effect of trichostatin A (TSA), a potent HDAC inhibitor, on the proliferation and migration of vascular smooth muscle cells (VSMCs), and we examined the role of reduced Trx1 levels in this effect.

Methods and results

TSA treatment time-dependently decreased Trx1 expression in rat VSMCs at both the mRNA and protein levels. It also enhanced platelet-derived growth factor (PDGF)-induced proliferation and migration of the VSMCs. By potentiating Akt phosphorylation, the siRNA-induced downregulation of Trx1 also enhanced VSMC proliferation and migration in respon

SUBMITTER: Song S 

PROVIDER: S-EPMC2791053 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature