Split-CreERT2: temporal control of DNA recombination mediated by split-Cre protein fragment complementation.

ABSTRACT:

Background

DNA recombination technologies such as the Cre/LoxP system advance modern biological research by allowing conditional gene regulation in vivo. However, the precise targeting of a particular cell type at a given time point has remained challenging since spatial specificity has so far depended exclusively on the promoter driving Cre recombinase expression. We have recently established split-Cre that allows DNA recombination to be controlled by coincidental activity of two promoters, thereby increasing spatial specificity of Cre-mediated DNA recombination. To allow temporal control of split-Cre-mediated DNA recombination we have now extended split-Cre by fusing split-Cre proteins with the tamoxifen inducible ERT2 domain derived from CreERT2.

Methodology/principal finding

SUBMITTER: Hirrlinger J 

PROVIDER: S-EPMC2791205 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature