A strategy for adenovirus vector targeting with a secreted single chain antibody.

ABSTRACT:

Background

Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an attractive means to achieve cell-specific targeting. However, a longstanding barrier to the development of Ad vectors with genetically incorporated scFvs has been the biosynthetic incompatibility between Ad capsid proteins and antibody-derived species. Specifically, scFv require posttranslational modifications not available to Ad capsid proteins due to their cytoplasmic routing during protein synthesis and virion assembly.

Methodology/principal findings

We have therefore sought to develop scFv-targeted Ad vectors using a secreted s

SUBMITTER: Glasgow JN 

PROVIDER: S-EPMC2791226 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature