Multidrug resistance protein 3 R652G may reduce susceptibility to idiopathic infant cholestasis.

ABSTRACT:

Aim

To evaluate the role of genetic factors in the pathogenesis of idiopathic infant cholestasis.

Methods

We performed a case-control study, including 78 infants with idiopathic infant cholestasis and 113 healthy infants as controls. Genomic DNA was extracted from peripheral venous blood leukocytes using phenol chloroform methodology. Polymerase chain reaction was used to amplify the multidrug resistance protein 3 (MDR3) R652G fragment, and products were sequenced using the ABI 3100 Sequencer.

Results

The R652G single nucleotide polymorphism (SNP) was significantly more frequent in healthy infants (allele frequency 8.0%) than in patients (allele frequency 2.60%) (P < 0.05), odds ratio, 0.29; 95% confidence interval, 0.12-0.84. The conjugated bilirubin in patients with

SUBMITTER: Chen XQ 

PROVIDER: S-EPMC2791281 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature