Use of high throughput sequencing to observe genome dynamics at a single cell level.

ABSTRACT: With the development of high throughput sequencing technology, it becomes possible to directly analyze mutation distribution in a genome-wide fashion, dissociating mutation rate measurements from the traditional underlying assumptions. Here, we sequenced several genomes of Escherichia coli from colonies obtained after chemical mutagenesis and observed a strikingly nonrandom distribution of the induced mutations. These include long stretches of exclusively G to A or C to T transitions along the genome and orders of magnitude intra- and intergenomic differences in mutation density. Whereas most of these observations can be explained by the known features of enzymatic processes, the others could reflect stochasticity in the molecular processes at the single-cell level. Our results demonstrate

SUBMITTER: Parkhomchuk D 

PROVIDER: S-EPMC2791585 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature