Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV.

ABSTRACT:

Background

The spike (S) protein is a major structural glycoprotein of coronavirus (CoV), the causal agent of severe acute respiratory syndrome (SARS). The S protein is a potent target for SARS-specific cell-mediated immune responses. However, the mechanism CoV pathogenesis in SARS and the role of special CTLs in virus clearance are still largely uncharacterized. Here, we describe a study that leads to the identification of a novel HLA-A*0201-restricted epitope from conserved regions of S protein.

Results

First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. Four of eight candidate peptides were tested by HLA-A*0201 binding assays. Among the four ca

SUBMITTER: Lv Y 

PROVIDER: S-EPMC2792222 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature