Using transcriptome profiling to characterize QTL regions on chicken chromosome 5.

ABSTRACT:

Background

Although many QTL for various traits have been mapped in livestock, location confidence intervals remain wide that makes difficult the identification of causative mutations. The aim of this study was to test the contribution of microarray data to QTL detection in livestock species. Three different but complementary approaches are proposed to improve characterization of a chicken QTL region for abdominal fatness (AF) previously detected on chromosome 5 (GGA5).

Results

Hepatic transcriptome profiles for 45 offspring of a sire known to be heterozygous for the distal GGA5 AF QTL were obtained using a 20 K chicken oligochip. mRNA levels of 660 genes were correlated with the AF trait. The first approach was to dissect the AF phenotype by identifying animal subgroups acc

SUBMITTER: Le Mignon G 

PROVIDER: S-EPMC2792231 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature