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Loop-mediated isothermal amplification for influenza A (H5N1) virus.

ABSTRACT: We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.

SUBMITTER: Jayawardena S

PROVIDER: S-EPMC2792861 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Loop-mediated isothermal amplification for influenza A (H5N1) virus.

Jayawardena Shanthi S Cheung Chung Y CY Barr Ian I Chan Kwok H KH Chen Honglin H Guan Yi Y Peiris J S Malik JS Poon Leo L M LL

Emerging infectious diseases 20070601 6

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes. ...[more]

PMID: 17553231

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