High throughput sequencing and proteomics to identify immunogenic proteins of a new pathogen: the dirty genome approach.

ABSTRACT:

Background

With the availability of new generation sequencing technologies, bacterial genome projects have undergone a major boost. Still, chromosome completion needs a costly and time-consuming gap closure, especially when containing highly repetitive elements. However, incomplete genome data may be sufficiently informative to derive the pursued information. For emerging pathogens, i.e. newly identified pathogens, lack of release of genome data during gap closure stage is clearly medically counterproductive.

Methods/principal findings

We thus investigated the feasibility of a dirty genome approach, i.e. the release of unfinished genome sequences to develop serological diagnostic tools. We showed that almost the whole genome sequence of the emerging pathogen Parachlamydia ac

SUBMITTER: Greub G 

PROVIDER: S-EPMC2793016 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature