Uracil misincorporation into DNA and folic acid supplementation.

ABSTRACT:

Background

Folate deficiency decreases thymidylate synthesis from deoxyuridylate, which results in an imbalance of deoxyribonucleotide that may lead to excessive uracil misincorporation (UrMis) into DNA during replication and repair.

Objective

We evaluated the relation between UrMis in different tissues and the effect of folate supplementation on UrMis.

Design

We analyzed UrMis concentrations in rectal mucosa (n = 92) and white blood cells (WBCs; n = 60) among individuals randomly assigned to receive supplementation with 1 mg folate/d or placebo, who were then evaluated for colorectal adenoma recurrence.

Results

As expected, total homocysteine was significantly lower among the study participants who received active folate treatment (Wilcoxon's P = 0.003) than a

SUBMITTER: Hazra A 

PROVIDER: S-EPMC2793107 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature