Monitoring the genomic stability of in vitro cultured rat bone-marrow-derived mesenchymal stem cells.

ABSTRACT: Bone-marrow-derived mesenchymal stem cells (MSCs) are multipotent cells capable of self-renewal and differentiation into multiple cell types. Accumulating preclinical and clinical evidence indicates that MSCs are good candidates to use as cell therapy in many degenerative diseases. For MSC clinical applications, an adequate number of cells are necessary so an extensive expansion is required. However, spontaneous immortalization and malignant transformation of MSCs after culture expansion have been reported in human and mouse, while very few data are present for rat MSCs (rMSCs). In this study, we monitored the chromosomal status of rMSCs at several passages in vitro, also testing the influence of four different cell culture conditions. We first used the conventional traditional cytogenetic

SUBMITTER: Foudah D 

PROVIDER: S-EPMC2793379 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature