Functional proteomics of failed filtering blebs.

ABSTRACT:

Purpose

To identify and determine the function of the proteins associated with failed filtering blebs following trabeculectomy.

Methods

Tenon's tissues, obtained during surgery for failed filtering blebs or obtained during cataract surgery on normal eyes, were analyzed by proteomics. The proteins showing significant differences between the two tissues were selected for identification by mass spectrometry. The location and expression pattern of ribosomal S6 kinase 2 (RSK2), one of the altered proteins, were determined. The effect of basic fibroblast growth factor (bFGF) on the expression pattern and function of RSK2 in NIH3T3 fibroblast cells was then investigated by an RNA knockdown technique.

Results

Eight proteins were found differentially expressed in failed filter

SUBMITTER: Kanamoto T 

PROVIDER: S-EPMC2793902 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature