TetR-type transcriptional regulator VtpR functions as a global regulator in Vibrio tubiashii.

ABSTRACT: Vibrio tubiashii, a causative agent of severe shellfish larval disease, produces multiple extracellular proteins, including a metalloprotease (VtpA), as potential virulence factors. We previously reported that VtpA is toxic for Pacific oyster (Crassostrea gigas) larvae. In this study, we show that extracellular protease production by V. tubiashii was much reduced by elevated salt concentrations, as well as by elevated temperatures. In addition, V. tubiashii produced dramatically less protease in minimal salts medium supplemented with glucose or sucrose as the sole carbon source than with succinate. We identified a protein that belongs to the TetR family of transcriptional regulators, VtpR, which showed high homology with V. cholerae HapR. We conclude that VtpR activates VtpA production bas

SUBMITTER: Hasegawa H 

PROVIDER: S-EPMC2794119 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature