Overlapping promoter targeting by Elk-1 and other divergent ETS-domain transcription factor family members.

ABSTRACT: ETS-domain transcription factors play important roles in controlling gene expression in a variety of different contexts; however, these proteins bind to very similar sites and it is unclear how in vivo specificity is achieved. In silico analysis is unlikely to reveal specific targets for individual family members and direct experimental approaches are therefore required. Here, we take advantage of an inducible dominant-negative expression system to identify a group of novel target genes for the ETS-domain transcription factor Elk-1. Elk-1 is thought to mainly function through cooperation with a second transcription factor SRF, but the targets we identify are largely SRF-independent. Furthermore, we demonstrate that there is a high degree of overlapping, cell type-specific, target gene bind

SUBMITTER: Boros J 

PROVIDER: S-EPMC2794171 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature