Cholesterol homeostasis in two commonly used human prostate cancer cell-lines, LNCaP and PC-3.

ABSTRACT:

Background

Recently, there has been renewed interest in the link between cholesterol and prostate cancer. It has been previously reported that in vitro, prostate cancer cells lack sterol-mediated feedback regulation of the major transcription factor in cholesterol homeostasis, sterol-regulatory element binding protein 2 (SREBP-2). This could explain the accumulation of cholesterol observed in clinical prostate cancers. Consequently, perturbed feedback regulation to increased sterol levels has become a pervasive concept in the prostate cancer setting. Here, we aimed to explore this in greater depth.

Methodology/principal findings

After altering the cellular cholesterol status in LNCaP and PC-3 prostate cancer cells, we examined SREBP-2 processing, downstream effects on promot

SUBMITTER: Krycer JR 

PROVIDER: S-EPMC2794383 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature