Proteomic detection of non-annotated protein-coding genes in Pseudomonas fluorescens Pf0-1.

ABSTRACT: Genome sequences are annotated by computational prediction of coding sequences, followed by similarity searches such as BLAST, which provide a layer of possible functional information. While the existence of processes such as alternative splicing complicates matters for eukaryote genomes, the view of bacterial genomes as a linear series of closely spaced genes leads to the assumption that computational annotations that predict such arrangements completely describe the coding capacity of bacterial genomes. We undertook a proteomic study to identify proteins expressed by Pseudomonas fluorescens Pf0-1 from genes that were not predicted during the genome annotation. Mapping peptides to the Pf0-1 genome sequence identified sixteen non-annotated protein-coding regions, of which nine were antisen

SUBMITTER: Kim W 

PROVIDER: S-EPMC2794547 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature