Global changes in processing of mRNA 3' untranslated regions characterize clinically distinct cancer subtypes.

ABSTRACT: Molecular cancer diagnostics are an important clinical advance in cancer management, but new methods are still needed. In this context, gene expression signatures obtained by microarray represent a useful molecular diagnostic. Here, we describe novel probe-level microarray analyses that reveal connections between mRNA processing and neoplasia in multiple tumor types, with diagnostic potential. We now show that characteristic differences in mRNA processing, primarily in the 3'-untranslated region, define molecular signatures that can distinguish similar tumor subtypes with different survival characteristics, with at least 74% accuracy. Using a mouse model of B-cell leukemia/lymphoma, we find that differences in transcript isoform abundance are likely due to both alternative polyadenylation

SUBMITTER: Singh P 

PROVIDER: S-EPMC2794997 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature