Cloning and pharmacological characterization of the dog P2X7 receptor.

ABSTRACT:

Background and purpose

Human and rodent P2X7 receptors exhibit differences in their sensitivity to antagonists. In this study we have cloned and characterized the dog P2X7 receptor to determine if its antagonist sensitivity more closely resembles the human or rodent orthologues.

Experimental approach

A cDNA encoding the dog P2X7 receptor was isolated from a dog heart cDNA library, expressed in U-2 OS cells using the BacMam viral expression system and characterized in electrophysiological, ethidium accumulation and radioligand binding studies. Native P2X7 receptors were examined by measuring ATP-stimulated interleukin-1beta release in dog and human whole blood.

Key results

The dog P2X7 receptor was 595 amino acids long and exhibited high homology (>70%) to the human an

SUBMITTER: Roman S 

PROVIDER: S-EPMC2795218 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature