Ontology highlight
ABSTRACT:
SUBMITTER: Christian TD
PROVIDER: S-EPMC2795520 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20091202 50
The catalytic mechanism of DNA polymerases involves multiple steps that precede and follow the transfer of a nucleotide to the 3'-hydroxyl of the growing DNA chain. Here we report a single-molecule approach to monitor the movement of E. coli DNA polymerase I (Klenow fragment) on a DNA template during DNA synthesis with single base-pair resolution. As each nucleotide is incorporated, the single-molecule Förster resonance energy transfer intensity drops in discrete steps to values consistent with ...[more]