Molecular characterization and phylogenetic analysis of the complete genome of a porcine sapovirus from Chinese swine.

ABSTRACT:

Background

Porcine sapovirus was first identified in the United States in 1980, hitherto, several Asian countries have detected this virus. In 2008, the first outbreak of gastroenteritis in piglets caused by porcine sapovirus in China was reported. The complete genome of the identified SaV strain Ch-sw-sav1 was sequenced and analyzed to provide gene profile for this outbreak.

Methods

The whole genome of Ch-sw-sav1 was amplified by RT-PCR and was sequenced. Sequence alignment of the complete genome or RNA dependent RNA polymerase (RdRp) gene was done. 3' end of ORF2 with 21-nt nucleotide insertion was further analyzed using software.

Results

Sequence analysis indicated that the genome of Ch-sw-sav1 was 7541 nucleotide long with two ORFs, excluding the 17 nucleotides pl

SUBMITTER: Yang S 

PROVIDER: S-EPMC2795755 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature