Quantitative biological imaging by ptychographic x-ray diffraction microscopy.

ABSTRACT: Recent advances in coherent x-ray diffractive imaging have paved the way to reliable and quantitative imaging of noncompact specimens at the nanometer scale. Introduced a year ago, an advanced implementation of ptychographic coherent diffractive imaging has removed much of the previous limitations regarding sample preparation and illumination conditions. Here, we apply this recent approach toward structure determination at the nanoscale to biological microscopy. We show that the projected electron density of unstained and unsliced freeze-dried cells of the bacterium Deinococcus radiodurans can be derived from the reconstructed phase in a straightforward and reproducible way, with quantified and small errors. Thus, the approach may contribute in the future to the understanding of the highly

SUBMITTER: Giewekemeyer K 

PROVIDER: S-EPMC2795774 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature