Amplification of coronary arteriogenic capacity of multipotent stromal cells by epidermal growth factor.

ABSTRACT:

Objective

We determined whether increasing adherence of multipotent stromal cells (MSCs) would amplify their effects on coronary collateral growth (CCG).

Methods and results

Adhesion was established in cultured coronary endothelials cells (CECs) or MSCs treated with epidermal growth factor (EGF). EGF increased MSCs adhesion to CECs, and increased intercellular adhesion molecule (ICAM-1) or vascular cell adhesion molecule (VCAM-1) expression. Increased adherence was blocked by EGF receptor antagonism or antibodies to the adhesion molecules. To determine whether adherent MSCs, treated with EGF, would augment CCG, repetitive episodes of myocardial ischemia (RI) were introduced and CCG was measured from the ratio of collateral-dependent (CZ) and normal zone (NZ) flows. CZ/NZ was

SUBMITTER: Belmadani S 

PROVIDER: S-EPMC2796538 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature