Cycle training increased GLUT4 and activation of mammalian target of rapamycin in fast twitch muscle fibers.

ABSTRACT:

Purpose

To determine whether cycle training of sedentary subjects would increase the expression of the principle muscle glucose transporters, six volunteers completed 6 wk of progressively increasing intensity stationary cycle cycling.

Methods

In vastus lateralis muscle biopsies, changes in expression of GLUT1, GLUT4, GLUT5, and GLUT12 were compared using quantitative immunoblots with specific protein standards. Regulatory pathway components were evaluated by immunoblots of muscle homogenates and immunohistochemistry of microscopic sections.

Results

GLUT1 was unchanged, GLUT4 increased 66%, GLUT12 increased 104%, and GLUT5 decreased 72%. A mitochondrial marker (cytochrome c) and regulators of mitochondrial biogenesis (peroxisome proliferator-activated receptor gamma c

SUBMITTER: Stuart CA 

PROVIDER: S-EPMC2796589 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature