Indoleamine 2,3-dioxygenase tissue distribution and cellular localization in mice: implications for its biological functions.

ABSTRACT: Earlier studies have suggested that indoleamine 2,3-dioxygenase (IDO) has a wide tissue distribution in mammals. However, detailed information on its cellular localization and also the levels of expression in various tissues is still scarce. In the present study, we sought to determine the cellular localization of IDO and also to quantify the level of its expression in various mouse tissues by using the branched DNA signal amplification assay, Western blotting, and immunohistochemical staining. The highest levels of constitutive IDO expression were found to be selectively present in the caput of epididymis, except for its initial segment. IDO expression was also detected inside the luminal compartment and even in the stereocilia within this region. In the prostate, high levels of IDO were

SUBMITTER: Dai X 

PROVIDER: S-EPMC2796611 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature